The history of DNA sequencing dates when Crick and Watson discovered the DNA structure in 1953. Richard Holley, in 1964, then performed the sequencing of the tRNA, which was the first attempt to sequence the nucleic acid.
Employing the technique of Holley and Walter, the two sequenced the genome of bacteriophage MS2. These sequenced molecules were the RNA, although the DNA sequencing was unperformed.
In 1977, Fredrick Sanger came up with the chain termination method, which was the method for sequencing DNA. In the same year, the chemical degradation method got explained by Walter Gilbert and Allan Maxam.
Since there was no automation, the chemical degradation and chain termination methods became more tedious and time-consuming. In 1986, however, Smith and Lorey developed the first semi-automated DNA method. Later on, in 1987, the Applied Bio-system had completely automated. In the following years, reliable, highly efficient, fast. And accurate next-generation sequencing platforms developed.
That discussed, what is DNA sequencing?
DNA sequencing is a laboratory technique used to discover the ideal DNA sequence. Both computational analysis and laboratory processing are significant processes in DNA sequencing. When the chemical reaction gets completed, the data generated gets sent to the computer laboratory.
Some of the different methods of DNA sequencing include:
- Sample preparation (DNA extraction)
- Data analysis
- Sequencing pre-prep
- DNA sequencing
- Amplicon’s purification
- PCR amplification of target sequence
- Sequencing pre-prep
Various methods of DNA sequencing are the Maxam and Gilbert method, Next-generation method, Chain termination method, automated method, clone by the clone sequencing method, the whole-genome shotgun sequencing method, pyrosequencing. And the semi-automated method.
Choosing the best DNA sequencing provider for you
When choosing a DNA sequence provider, you want to consider a provider with proven technology. Such providers ensure high-quality sequencing data, making discoveries that were unimaginable a few years ago possible.
You also want to consider a DNA sequence provider with a proven track record of availing reliable solutions, empowering researchers to perform studies throughout, and offering reasonable prices for their services.
You want to work with a provider who goes the extra mile to get you results. And has experts to ensure accurate results.
Applications of DNA sequencing
DNA sequencing can get applied in various industries and purposes. These includes:
In the medical field, DNA sequencing identifies genes that result in hereditary disorders. And also detect new mutations. The sequencing techniques have also played a significant role in oncology and cancer studies to identify cancer-causing genes. And in the discovery of defective genes to replace them with healthy ones.
In the forensic field, DNA sequencing can get used for parental verification. And identification of individuals through samples such as hair, blood, tissue, or nails. And during a criminal investigation to provide proof at a crime scene.
In the agricultural sector, DNA sequencing helps in the identification of GMO species. Any minor variations with the agricultural products are closely monitored, including assessing the resistance of various crops to insects and crops. DNA sequencing has also been helpful in the production of healthy livestock, leading to high-quality milk and meat.
DNA sequencing can also get used in constructing maps such as genome maps, restriction digestion maps, and chromosomal maps. It can also get used in intron/exon, identification, and detection. And in the study of new bacterial species.
The sequencing technique helps to eliminate the time-consuming and culturing methods. With this, scientists can also effortlessly identify new strains and mutations.
The advancement in evolutionary studies has also been made possible by DNA sequencing. That is because various genes and sequences can get generated. And new variations through evolutions created.
Finally, DNA sequencing is also applicable in the studying of the asymptomatic high-risk population. That helps to detect and take preventative measures before the occurrence of a disease.